Product information "Cas9 Expressing MDA-MB-231 cell pool"
Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9 expressing MDA-MB-231 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Keywords: | SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1 |
Supplier: | BPS Bioscience |
Supplier-Nr: | 78069 |
Properties
Application: | Quick knock-out cell pool / cell line generation, SgRNA screening |
Reactivity: | Human |
Handling & Safety
Storage: | Liquid nitrogen (store in liquid nitrogen) |
Shipping: | -80°C (International: °C) |
Caution Our products are for laboratory research use only: Not for administration to humans!