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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Biomol/U-87 MG Whole Cell Lysate/500 µg/W09-001-GX2
产品编号:W09-001-GX2
市  场 价:¥3280.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$164.00
品      牌: Biomol
公      司:Biomol
公司分类:
Biomol/U-87 MG Whole Cell Lysate/500 µg/W09-001-GX2
商品介绍
Product information "U-87 MG Whole Cell Lysate"
U-87 MG cells were grown in Eagle"s Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified BCA assay using a commercially available kit. Protein concentration was adjusted to 1 mg/mL using 2X SDS-PAGE Sample buffer. Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.  All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Supplier: Rockland Immunochemicals
Supplier-Nr: W09-001-GX2

Properties

Application: WB
Host: Human
Reactivity: Human
Format: Liquid

Database Information

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution Our products are for laboratory research use only: Not for administration to humans!
品牌介绍

五十多年来,Biomol一直是德国生命科学和生物技术产品的领先供应商之一。我们对生命科学研究的复杂理解从根本上与以下事实相关:我们公司是在同一时间成立的,现代生物医学时代已经开始。1960年代后期。

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